Composite

Part:BBa_K3089026:Design

Designed by: Aiai Dong   Group: iGEM19_Greatbay_SCIE   (2019-10-16)


T7 promoter+rBalcp19K-linker-mfp5-His


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
    Illegal PstI site found at 411
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 720
    Illegal XhoI site found at 527
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
    Illegal PstI site found at 411
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
    Illegal PstI site found at 411
    Illegal AgeI site found at 390
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Linkers are applied to prevent components jamming into each other.


Source

rBalcp19k - barnacle cement protein Mfp5 - mussel foot protein, Mytilus galloprovincialis

References