Composite
Part:BBa_K3089026:Design
Designed by: Aiai Dong Group: iGEM19_Greatbay_SCIE (2019-10-16)
T7 promoter+rBalcp19K-linker-mfp5-His
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 47
Illegal PstI site found at 411 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 411
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 720
Illegal XhoI site found at 527 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 47
Illegal PstI site found at 411 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 47
Illegal PstI site found at 411
Illegal AgeI site found at 390 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Linkers are applied to prevent components jamming into each other.
Source
rBalcp19k - barnacle cement protein Mfp5 - mussel foot protein, Mytilus galloprovincialis